Spatial Screening of Cyclic Neoglycopeptides: Identification of Polyvalent Wheat-Germ Agglutinin Ligands
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چکیده
منابع مشابه
Spatial screening of cyclic neoglycopeptides: identification of polyvalent wheat-germ agglutinin ligands.
Natural and synthetic polyvalent ligands can act as inhibitors of biological processes or as effectors of signal-transduction pathways. 2] In the case of carbohydrate–lectin interactions, polyvalency is of particular importance because individual carbohydrate epitopes are usually bound with only weak affinity and often broad specificity. The potency of a polyvalent inhibitor depends on the spat...
متن کاملSpatial Screening of Cyclic Neoglycopeptides: Identification of Multivalent Wheat Germ Agglutinin Ligands**
General. Solid-phase peptide synthesis was carried out in plastic syringe reactors. Fmocprotected amino acids were obtained from Novabiochem (Läufelfingen, Switzerland) or prepared according to standard procedures. Bovine serum albumin (A7638), biotinylated WGA (L5142), alkaline phosphatase-coupled monoclonal anti biotin antibody (A6561), BCIP/NBT tablets (B5655), peroxidase-labeled WGA (L7017)...
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Multivalent carbohydrate-protein interactions are frequently involved in essential biological recognition processes. Accordingly, multivalency is often also exploited for the design of high-affinity lectin ligands aimed at the inhibition of such processes. In a previous study (D. Schwefel et al., J. Am. Chem. Soc. 2010, 132, 8704-8719) we identified a tetravalent cyclopeptide-based ligand with ...
متن کاملWheat Germ Agglutinin
Procedures for the isolation, purification, and crystallization of wheat germ agglutinin are described. The agglutinin was purified 184-fold to homogeneity from commercial wheat germ lipase. A molecular weight of 23,500 was estimated for the protein by means of sedimentation equilibrium and sodium dodecyl sulfate gel electrophoresis. The agghxtinin is a glycoprotein. Amino acid and carbohydrate...
متن کاملImmunocytochemical localization of wheat germ agglutinin in wheat
Immunocytological techniques were developed to localize the plant lectin, wheat germ agglutinin (WGA), in the tissues and cells of wheat plants. In a previous study we demonstrated with a radioimmunoassay that the lectin is present in wheat embryos and adult plants both in the roots and at the base of the stem. We have now found, using rhodamine, peroxidase, and ferritin-labeled secondary antib...
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ژورنال
عنوان ژورنال: Angewandte Chemie International Edition
سال: 2004
ISSN: 1433-7851,1521-3773
DOI: 10.1002/anie.200352055